BDBM25817 US9144538, Fluconazole Fluconazole US11247981, Example Fluconazole US10221160, Fluconazole US9556143, Fluconazole US9221791, Fluconazole 2-(2,4-difluorophenyl)-1,3-bis(1H-1,2,4-triazol-1-yl)propan-2-ol US9138393, Fluconazole Diflucan Triflucan CHEMBL106 cid_3365 Fluconazole (f) Fungata
- PubChem, PC Fluorescence Cell-Based Secondary Assay to Measure Toxicity of Compounds Not in the Presence of Fluconazole PubChem Bioassay (2010)
- PubChem, PC Fluorescence Cell-Based Secondary Assay of Resistant C. albicans Growth in the Presence of Fluconazole PubChem Bioassay (2010)
- PubChem, PC Fluorescence Cell-Based Secondary Assay to Identify Inhibitors of Resistant C. albicans Growth in the Presence of Fluconazole PubChem Bioassay (2010)
- Dao, VH; Ourliac-Garnier, I; Bazin, MA; Jacquot, C; Baratte, B; Ruchaud, S; Bach, S; Grovel, O; Le Pape, P; Marchand, P Benzofuro[3,2-d]pyrimidines inspired from cercosporamide CaPkc1 inhibitor: Synthesis and evaluation of fluconazole susceptibility restoration. Bioorg Med Chem Lett 28: 2250-2255 (2018)
- Yin, W; Zhang, Y; Cui, H; Jiang, H; Liu, L; Zheng, Y; Wu, T; Zhao, L; Sun, Y; Su, X; Li, S; Zhao, D; Cheng, M Design, synthesis and evaluation of novel 5-phenylthiophene derivatives as potent fungicidal of Candida albicans and antifungal reagents of fluconazole-resistant fungi. Eur J Med Chem 225: (2021)
- Fluorescence Cell-Based Secondary Assay of Resistant C. albicans Growth in the Presence of Fluconazole Broad Institute: Reversing Antifungal Drug Resistance Project ID: 2037 Keywords: Candida albicans, drug resistance, fluconazole, Hsp90, Calcineurin, stress response Primary Collaborators: Susan Lindquist, Whitehead Institute for Biomedical Research, sll@wi.mit.edu Assay Overview: The basic assay strategy will consist of highly fluconazole-resistant C. albicans clinical isolate cultured in 384-well format in the presence of a sub-toxic concentration of fluconazole. Test compounds that inhibit subsequent growth in the presence of fluconazole will merit further evaluation for their synergy with fluconazole. This whole cell phenotypic screening approach will only capture compounds that retain activity in biological media and are capable of entering and accumulating in fungi to bioactive concentrations. Grossly cytotoxic compounds will be removed through subsequent counterscreens. Compound activity will be measured by the metabolism of Alamar Blue, a cell stain that is metabolized to
- Fluorescence Cell-Based Secondary Assay to Identify Inhibitors of Resistant C. albicans Growth in the Presence of Fluconazole Broad Institute: Reversing Antifungal Drug Resistance Project ID: 2037 Keywords: Candida albicans, drug resistance, Fluconazole, Hsp90, Calcineurin, stress response Primary Collaborators: Susan Lindquist, Whitehead Institute for Biomedical Research, sll@wi.mit.edu Assay Overview: The basic assay strategy will consist of highly Fuconazole-resistant C. albicans clinical isolate cultured in 384-well format in the presence of a sub-toxic concentration of Fluconazole. Test compounds that inhibit subsequent growth in the presence of Fluconazole will merit further evaluation for their synergy with Fluconazole. This whole cell phenotypic screening approach will only capture compounds that retain activity in biological media and are capable of entering and accumulating in fungi to bioactive concentrations. Grossly cytotoxic compounds will be removed through subsequent counterscreens. Compound activity will be measured by the metabolism of Alamar Blue, a cell stain that is metabolized to a
- Fluorescence Cell-Based Secondary Assay to Measure Toxicity of Compounds Not in the Presence of Fluconazole Keywords: Candida albicans, drug resistance, Fluconazole, Hsp90, Calcineurin, stress response Primary Collaborators: Susan Lindquist, Whitehead Institute for Biomedical Research, sll@wi.mit.edu Assay Overview: The basic assay strategy will consist of NIH 3T3 mammalian fibroblasts cultured in 384-well format in the presence of a sub-toxic concentration of Fluconazole. Test compounds that do not inhibit subsequent growth in the presence of Fluconazole will merit further evaluation for their non-toxicity. This whole cell phenotypic screening approach will only capture compounds that are capable of entering and accumulating in cells to bioactive concentrations. Compound activity will be measured by the metabolism of Alamar Blue, a cell stain that is metabolized to a fluorescent product by living cells but that remains non-fluorescent in wells with growth-inhibited organisms. Probe attributes: a. Compounds that inhibit yeast growth in the presence, but not in the absence of 8 ug/ ml Fl
- Luminescence Cell-Based Secondary Assay to Identify Inhibitors of Hsp90 Keywords: Candida albicans, drug resistance, Fluconazole, Hsp90, stress response Assay Overview: Method for determining if compound acts as Hsp90 inhibitor. Saccharomyces strain expressing B-galactosidase driven by glucocorticoid response element is exposed to compounds. Loss of signal indicates interference with hsp90 function. Expected Outcome: Reduction of signal indicates hsp90 inhibition by compound.
- Luminesence Cell-Based Secondary Assay to Identify Inhibitors of Hsp90 Keywords: Candida albicans, drug resistance, Fluconazole, Hsp90, stress response Assay Overview: Method for determining if compound acts as Hsp90 inhibitor. Saccharomyces strain expressing B-galactosidase driven by glucocorticoid response element is exposed to compounds. Loss of signal indicates interference with hsp90 function. Expected Outcome: Reduction of signal indicates hsp90 inhibition by compound.
- Fluorescence Cell-Based Secondary Assay to Identify Inhibitors of Calcineurin Keywords: Candida albicans, drug resistance, Fluconazole, calcineurin, stress response Assay Overview: Method for determining if compound acts as Calcineurin inhibitor. S cerevisiae expressing beta-galactosidase driven by 4 tandem copies of the calcineurin-dependent response element (CDRE) is exposed to compounds followed by challenge with CaCl2 stressor. Potential inhibition of calcineurin function will be evaluated by loss of signal. Expected Outcome: Reduction of signal indicates calcineurin inhibition by compound.
- Luminesence Cell-Based Secondary Assay to Identify Inhibitors of Calcineurin Keywords: Candida albicans, drug resistance, Fluconazole, calcineurin, stress response Assay Overview: Method for determining if compound acts as Calcineurin inhibitor. S cerevisiae expressing beta-galactosidase driven by 4 tandem copies of the calcineurin-dependent response element (CDRE) is exposed to compounds followed by challenge with CaCl2 stressor. Potential inhibition of calcineurin function will be evaluated by loss of signal. Expected Outcome: Reduction of signal indicates calcineurin inhibition by compound.